Galacto-oligosaccharides improve barrier function and relieve colonic inflammation via modulating mucosa-associated microbiota composition in lipopolysaccharides-challenged piglets

Abstract Background Galacto-oligosaccharides (GOS) have been shown to modulate the intestinal microbiota of suckling piglets exert beneficial effects on function. However, modulation and function by GOS in inflammation injury models has rarely reported. In this study, we investigated colonic mucosal composition, barrier inflammatory response lipopolysaccharides (LPS)-challenged piglets. Methods A total 18 newborn were divided into three groups, CON group, LPS-CON group LPS-GOS group. Piglets orally fed with 1 g/kg body weight solution every day. On d 14, challenged intraperitoneally LPS solution. All slaughtered 2 h after intraperitoneal injection sampled. Results We found that mucosa LPS-challenged was significantly injured shedding, while maintained its structure. Moreover, reduced concentration malondialdehyde (MDA) activity reactive oxygen species (ROS) piglets, increased antioxidant capacity (T-AOC). relative abundance norank_f__Muribaculaceae Romboutsia , decreased Alloprevotella Campylobacter Helicobacter addition, concentrations acetate, butyrate short chain fatty acids (SCFAs) digesta interleukin 1β (IL-1β), 6 (IL-6), tumor necrosis factor-α (TNF-α) cluster differentiation 14 (CD14), mRNA expression Toll-like receptor 4 ( TLR4 ) myeloid primary 88 MyD88 mucin2 MUC2 ), protein Claudin-1 zonula occluden-1 (ZO-1) Conclusions These results suggested can mucosa-associated composition improve